|
KMID : 0525619980030030228
|
|
1998 Volume.3 No. 3 p.228 ~ p.232
|
|
|
Separation and Characterization of Water - Soluble Oligomers from the Reaction Mixture of Chitosan Treated with Chitosanolytic Enzymes
|
|
한범구/Han BK
이우진/조도현/Lee WJ/Jo DH
|
|
|
Abstract
|
|
|
|
Chitosan with 80% deaceylation and 30cps of viscosity was treated with papain, commercial chitosanase from Bacillus sp.(B-chitosanase), and a chitosanase from the digestive tract of broiler(G-chitosanase). Water-soluble chitosan oligomers were separated from the reaction mixture by differential precipitation and further analyzed by gel filteration. The conversion ratio of chitosan into water-soluble chitosan oligomers was 26% for B-chitosanase, 33% for G-chitosanase, and 39% for papain. With gel filteration of the separated oligomers, the major constituent was identified as dimer of glucosamine for B-chitosanase and papain and the trimer for G-chitosanase.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|